论文部分内容阅读
目的:研究fMLP刺激人多形核白细胞产生活性氧和一氧化氮的动力学过程并评价川芎嗪的作用。方法:实验于2001年在中国科学院生物物理所细胞生物实验室完成。应用超微弱化学发光、电子自旋共振自旋捕集技术和自旋探针氧测定法,测定多形核白细胞呼吸爆发过程中产生的活性氧、一氧化氮及其氧消耗。结果:fMLP刺激人多形核白细胞产生的化学发光既与一氧化氮合成酶合成一氧化氮有关,又与NADPH氧化酶催化产生超氧阴离子有关。化学发光动力学过程呈双峰曲线。第二峰总比第一峰晚几分钟。第一峰主要与超氧阴离子的产生有关,第二峰则与一氧化氮合成与释放有关,川芎嗪能直接清除超氧阴离子,抑制多形核白细胞诱导型一氧化氮合成酶合成一氧化氮。结论:人多形核白细胞激活后产生活性氧及一氧化氮的次序有先后,二者可迅速反应生成过氧化亚硝基,而川芎嗪是一种有效一氧化氮合酶活性调节剂,也是一种较强的抗氧化剂。
OBJECTIVE: To investigate the kinetics of the production of reactive oxygen species and nitric oxide by fMLP-stimulated human polymorphonuclear leukocytes and to evaluate the effects of tetramethylpyrazine. METHODS: Experiments were performed at the Cell Biology Laboratory, Institute of Biophysics, Chinese Academy of Sciences in 2001. Using ultraweak chemiluminescence, electron spin resonance spin trapping, and spin-probe oxygen determination, reactive oxygen species, nitric oxide, and oxygen consumption produced during respiratory burst of polymorphonuclear leukocytes were measured. RESULTS: The chemiluminescence produced by fMLP-stimulated human polymorphonuclear leukocytes was related to the synthesis of nitric oxide by nitric oxide synthase and the production of superoxide anion catalyzed by NADPH oxidase. The chemiluminescence kinetics show a bimodal curve. The second peak is always a few minutes later than the first peak. The first peak is mainly related to the production of superoxide anion, and the second peak is related to the synthesis and release of nitric oxide. Ligustrazine can directly remove superoxide anion and inhibit the synthesis of nitric oxide by polymorphonuclear leukocyte inducible nitric oxide synthase. . CONCLUSIONS: The order of production of reactive oxygen species and nitric oxide after activation of human polymorphonuclear leukocytes is sequential. Both can rapidly react to form peroxynitrite, and tetramethylpyrazine is a potent nitric oxide synthase modulator. A stronger antioxidant.