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通过测定不同花色瓜叶菊舌状花花色表型和定性定量分析其花青苷成分组成,探讨不同瓜叶菊花色表型与其所含花青素类色素类型之间的关系。采用分光色差计(NF333)测量了不同花色的色相值。用高效液相色谱—光电二极管阵列检测技术(HPLC-PAD)和高效液相色谱—电喷雾离子化—质谱联用技术(HPLC-ESI-MS)分析花瓣中花青苷和黄酮醇的组成及含量。分析表明:花色表型中亮度和花青苷总量之间存在线性负相关。蓝色和红色瓜叶菊花色分别由飞燕草素苷元(Dp)和矢车菊素苷元(Cy)为核心的花青苷决定。粉色瓜叶菊含有Cy和天竺葵素苷元(Pg)为核心的花青苷。紫色瓜叶菊主要含有Dp和Cy为核心的花青苷。瓜叶菊花色亮度与花青苷含量负相关,瓜叶菊花红色程度和Cy为核心的花青苷含量正相关。瓜叶菊主要由花青素决定其呈色。
The anthocyanin compositions were determined by measuring the flower color phenotypes of the tongue-flower of Cucurbitaceae and the relationship between the anthocyanin phenotypes and the flower color phenotypes. The hue values of different suit colors were measured using a spectrocolorimeter (NF333). The composition of anthocyanins and flavonols in petals were analyzed by HPLC-PAD and HPLC-ESI-MS. content. The analysis shows that there is a linear negative correlation between brightness and total anthocyanin in the flower color phenotype. Blue and red cineraria colors were determined by anthocyanins with delphinidin (Dp) and cyanidin (Cy) as the core. Pink cinerea contains Cy and anthocyanin aglycone (Pg) as the core of anthocyanins. Purple cineraria mainly contains Dp and Cy as the core anthocyanins. Cucumber leaf flower color brightness and anthocyanin content negatively correlated, Cucurbitaceae red degree and Cy core anthocyanin content was positively correlated. Cineraria is predominantly anthocyanidin-colored.