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研究3种中草药的水提物对北虫草发酵过程中菌丝量和活性产物产量的影响。将不同剂量的3种中草药水提物分别加入到发酵基础培养基中,培养7 d后检测北虫草生物量和活性成分含量。结果表明,甘草、五味子在添加量为15 mg/m L,刺五加的添加量为10 mg/m L时,不仅可促进北虫草细胞的生长(P<0.01),还对北虫草菌丝体及发酵液中腺苷及虫草素的形成都有显著促进作用(P<0.01)。本研究结果为北虫草今后的生产和开发提供一定的理论基础。
The effects of aqueous extracts of three kinds of Chinese herbal medicines on mycelium yield and active product yield during the fermentation of Cordyceps militaris were studied. The different concentrations of three kinds of Chinese herbal medicine aqueous extracts were added to the fermentation basal medium, cultured 7 d after the detection of Cordyceps biomass and active ingredient content. The results showed that licorice root and Schisandra chinensis could not only promote the growth of Cordyceps militaris cells at the dosage of 15 mg / m L and Acanthopanax senticosus addition of 10 mg / m L (P <0.01) Body and fermentation broth adenosine and cordycepin formation have a significant role in promoting (P <0.01). The results of this study provide some theoretical basis for the future production and development of Cordyceps militaris.