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目的 :探讨嗜酸性细胞阳离子蛋白 (ECP)和白细胞介素 5 (IL 5 )与哮喘发病的关系 ,并评价诱导痰在哮喘研究中的作用。方法 :采用高渗盐水雾化诱导痰液 ,以荧光免疫法和酶联免疫法对哮喘组 ,慢性喘息型支气管炎组 ,健康组血清和诱导痰中ECP、IL 5水平进行同步检测并测定肺功能 (FEV1 /FVC)。结果 :大部分患者 (96 % )能耐受诱导痰检查。哮喘组急性期血清和诱导痰中不仅ECP及IL 5水平较其他各组明显升高 ,而且ECP与IL 5水平之间存在显著正相关性 ,ECP或IL 5与FEV1 /FVC呈显著负相关性。结论 :ECP和IL 5在哮喘发病中起着十分重要的作用 ,诱导痰检查具有安全、简便、结果重复性强的优点 ,用于哮喘的研究具有较大价值。
Objective: To investigate the relationship between eosinophil cationic protein (ECP) and interleukin 5 (IL 5) and the pathogenesis of asthma and to evaluate the role of induced sputum in the study of asthma. Methods: The sputum was induced by hypertonic saline atomization. The levels of ECP and IL-5 in the asthmatic group, the chronic asthmatic bronchitis group, the healthy group and the induced sputum were detected by fluorescence immunoassay and enzyme-linked immunosorbent assay Function (FEV1 / FVC). Results: Most patients (96%) were able to tolerate induced sputum examination. The levels of ECP and IL-5 in serum and induced sputum of acute asthma group were significantly higher than those of other groups, and there was a significant positive correlation between ECP and IL-5 level, and a significant negative correlation between ECP or IL-5 and FEV1 / FVC . Conclusion: ECP and IL 5 play an important role in the pathogenesis of asthma. Induced sputum examination is safe, simple and reproducible. It is of great value in the study of asthma.