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目的:研究金属蛋白酶解离素28(a disintegrin and metalloproteinase 28,ADAM28)反义核酸(antisense oligodeoxynucleotide,AS-ODN)对人牙龈成纤维细胞(human gingival fibroblasts,HGFs)增殖、分化、凋亡的影响,并分析可能的作用机制。方法:采用细胞培养、基因转染、四甲基唑蓝(MTT)比色法、酶动力学法和流式细胞术(FCM)检测ADAM28反义核酸转染HGFs后对细胞生物学特性的影响。采用SPSS16.0软件包中的SNK检验对数据进行统计学分析。结果:ADAM28 AS-ODN组HGFs的增殖活性显著降低。AS-ODN组HGFs处于S期的细胞百分比显著低于SODN组和未转染组,G2+M期的细胞百分比显著低于未转染组,AS-ODN组的细胞增殖指数(PI=S+G2M)显著低于其他2组,差异显著。AS-ODN组碱性磷酸酶(ALP)分泌活性、凋亡细胞百分比显著升高。结论:ADAM28反义核酸可显著抑制HGFs的增殖,并影响细胞周期的变化,促进其分化,显著诱导HGFs的凋亡。
AIM: To investigate the effect of ADAM28 antisense oligodeoxynucleotide (AS-ODN) on the proliferation, differentiation and apoptosis of human gingival fibroblasts (HGFs) , And analyze the possible mechanism of action. Methods: The effects of ADAM28 antisense oligonucleotide on HGFs were detected by cell culture, gene transfection, MTT colorimetric assay, enzyme kinetic assay and flow cytometry (FCM) . The data were statistically analyzed using the SNK test in the SPSS16.0 software package. Results: The proliferative activity of HGFs in ADAM28 AS-ODN group was significantly decreased. The percentages of HGFs in S phase in AS-ODN group were significantly lower than that in SODN group and non-transfected group, the percentage of cells in G2 + M phase was significantly lower than that in non-transfected group and AS-ODN group (PI = S + G2M) was significantly lower than the other two groups, the difference was significant. AS-ODN group alkaline phosphatase (ALP) secretion activity, the percentage of apoptotic cells was significantly increased. Conclusion: ADAM28 antisense oligonucleotide can significantly inhibit the proliferation of HGFs, affect the cell cycle, promote the differentiation and induce the apoptosis of HGFs significantly.