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本文就可可子叶、叶片及茎段体细胞胚状体离体诱导及发育的标准化技术和培养基进行了初步探讨。以将近成熟种子的子叶为外植体,在附加0.5mg/lNAA+0.5mg/lBAP+椰子水(15%)的MS培养上可以最大程度地诱导出能大量发生体细胞胚胎的愈伤组织。至于叶片及茎段,则仅能诱导出非胚性愈伤组织。分离并培养于相同成分液体培养基上(使用滤纸桥)的体细胞胚状体会发育出一条明显的在其顶端带有一冠状覆盖物的主根。当转移到含0.5mg/l玉米素+0.1mg/l激动素的培养基上其主根开始伸长时,该冠状覆盖物则脱落。
In this paper, the standardization techniques and medium for in vitro induction and development of somatic embryoid bodies in cotyledons, leaves and stems of cocoa were discussed. Using cotyledons of nearly mature seeds as explants, callus, which can generate somatic embryos in large quantities, can be induced to the greatest extent on MS culture supplemented with 0.5 mg / l NAA + 0.5 mg / l BAP + coconut water (15%). As for the leaves and stem segments, only non-embryogenic callus can be induced. Somatic embryos isolated and cultured on liquid broths of the same composition (using a filter paper bridge) develop a distinct main root with a crown covering at the top. The crown cover shed when the main root began to elongate when it was transferred to a medium containing 0.5 mg / l zeatin + 0.1 mg / L kinetin.