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本文报道三倍体回交杂种的流式细胞仪测试方法和结果。取正常二倍体鲤鲫杂交鱼的体细胞液进样所得直方图为标准 ,通过对 36尾鲤鲫杂交回交种和其雌核发育子代的细胞核DNA相对含量的测定 ,观察到杂种F1 雌性回交子代中产生的三倍体和非整倍三倍体 (2～ 3倍体之间 )现象 ,测试结果以直方图表示
This paper reports the method and results of flow cytometry of triploid backcross hybrids. Histograms of somatic cell aspirates from hybrid diploid carp crucian carp were used as the standard. The relative content of nuclear DNA in the hybridized backcross of 36 carp crucian and its progeny of female gynogenetic hybrid was determined. Backcross progeny generated triploid and aneuploidy triploid (2 to 3 ploidy) phenomenon, the test results to a histogram