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目的检测缺氧诱导因子-1α(HIF-1α)、血管内皮生长因子-C(VEGF-C)在卵巢上皮性癌组织中的表达,分析HIF-1α和VEGF-C的表达与卵巢上皮性癌淋巴管生成及癌进展之间的关系。方法采用免疫组化SP法和Western印迹法对70例卵巢上皮性癌、30例良性卵巢肿瘤及正常卵巢组织进行HIF-1α、VEGF-C检测;以D2-40为淋巴管内皮特异性标记物,进行微淋巴管密度(MLVD)测定,并分析他们与卵巢上皮性癌临床病理学特征的关系。结果 HIF-1α、VEGF-C在卵巢癌组织中的表达明显高于正常卵巢及良性卵巢肿瘤组织(均P<0.05);MLVD计数在卵巢癌组中明显高于良性卵巢肿瘤组(P<0.05)。卵巢癌组中,HIF-1α、VEGF-C的表达及D2-40标记的MLVD计数均随临床分期及肿瘤组织学分级的增加而升高(均P<0.05)。卵巢癌组中,HIF-1α、VEGF-C的表达分别与MLVD呈正相关,且HIF-1α和VEGF-C的表达亦呈正性相关。结论 HIF-1α及VEGF-C的高表达促进了卵巢上皮性癌组织中淋巴管的形成及淋巴转移,并与卵巢癌临床病理学特征关系密切。
Objective To detect the expression of hypoxia inducible factor-1α (HIF-1α) and vascular endothelial growth factor-C (VEGF-C) in ovarian epithelial carcinoma and to analyze the relationship between the expression of HIF-1α and VEGF-C and ovarian epithelial carcinoma Lymphangiogenesis and the relationship between cancer progression. Methods Immunohistochemical SP method and Western blotting were used to detect HIF-1α and VEGF-C in 70 cases of epithelial ovarian cancer, 30 cases of benign ovarian tumor and normal ovarian tissue. D2-40 was used as lymphatic endothelial specific marker, Lymphatic vessel density (MLVD) measurements were performed and their relationship to clinicopathological features of epithelial ovarian cancer was analyzed. Results The expression of HIF-1α and VEGF-C in ovarian cancer tissues was significantly higher than that in normal ovarian and benign ovarian tumor tissues (all P <0.05). The MLVD count in ovarian cancer group was significantly higher than that in benign ovarian tumor group (P <0.05) ). In ovarian cancer group, the expression of HIF-1α and VEGF-C and the count of D2-40 labeled MLVD increased with the increase of clinical stage and tumor histological grade (all P <0.05). In ovarian cancer group, the expression of HIF-1α and VEGF-C was positively correlated with MLVD, and the expression of HIF-1α and VEGF-C were also positively correlated. Conclusion The high expressions of HIF-1α and VEGF-C promote lymphangiogenesis and lymphatic metastasis in ovarian epithelial carcinoma and are closely related to clinicopathological features of ovarian cancer.