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目的 用 3种不同植瘤方式建立稳定的兔Vx 2移植性肝癌模型 ,分析移植性肝肿瘤的DSA影像特征。方法 6 0只新西兰白兔随机分成 3组 ,每组 2 0只。第 1组 ,将Vx 2瘤细胞 (约 5× 10 7个 )经肝动脉灌注入兔的肝脏 ;第 2组 ,将Vx 2瘤细胞 (约 5× 10 7个 )经剖腹途径接种于兔的肝左叶 ;第3组 ,将瘤组织块 (约含 10 6～ 10 8个瘤细胞 )经剖腹途径植入兔肝左叶。之后 ,对 3组兔比较观察 :1.不同方式植瘤的成活率 ;2 .肝内肿瘤体积变化和肿瘤生长率 ;3.大体及镜下 (光镜和电镜 )瘤组织形态特征 ;4 .成熟模型的DSA表现。结果 1.3组植瘤成活率分别为 7/ 2 0、10 / 2 0、19/ 2 0 ,第 3组植瘤成活率最高 (P <0 .0 5 ) ,其瘤体呈指数性生长 ;2 .病理学及CT表明该瘤在肝组织中呈浸润式生长 ,其性状与移植于兔其它部位的Vx 2鳞状细胞癌特征相似 ;3.DSA影像示移植性肝肿瘤具有丰富的血供。结论 成功建立了兔Vx 2移植性肝癌模型 ,瘤组织块种植方式成功率明显高于其他两种方法 ,为肝癌介入治疗的实验研究提供了可靠的大型动物模型
Objective To establish a stable rabbit Vx 2 transplanted hepatocellular carcinoma (HCC) model using three different tumor types and analyze the DSA features of transplanted liver tumors. Methods Sixty New Zealand white rabbits were randomly divided into three groups of 20 rats. In group 1, Vx2 tumor cells (about 5 × 10 7 cells) were perfused into the liver of rabbits via the hepatic artery. In group 2, Vx 2 tumor cells (about 5 × 10 7 cells) were inoculated into rabbits by cesarean section Left lobe of the liver; Group 3, the tumor mass (about 106 to 108 tumor cells) was implanted into the left lobe of rabbit liver via cesarean section. After 3 groups of rabbits were compared: 1. The survival rate of different ways of tumor; 2. Intrahepatic tumor volume changes and tumor growth rate; 3. General and microscopic (light and electron microscopy) tumor morphological features; DSA performance of mature models. Results The survival rate of tumor in group 1.3 was 7 / 20,10 / 20,19 / 20 respectively, and the survival rate in group 3 was the highest (P <0.05). The tumor growth rate was exponential. 2 .The pathology and CT showed that the tumor was infiltrative in liver tissue and its characteristics were similar to those of Vx 2 squamous cell carcinoma transplanted in other parts of the rabbit.3.The DSA image showed that the transplanted liver tumor had abundant blood supply. Conclusion The model of Vx 2 transplanted liver cancer in rabbits was established successfully. The success rate of tumor tissue mass implantation was significantly higher than that of the other two methods, which provided a reliable large animal model for the experimental study of interventional therapy of liver cancer