本文描述了一种筛选检测小肠结肠炎耶尔森氏菌有毒和无毒克隆的简单方法。该菌毒力株在RPMI 1640琼脂(含40mM HEPES和1.5%纯琼脂),形成大型和小型菌落。通过自身凝集试验(一种毒力标记)检测,小型菌落常常阳性,而大型菌落为阴性。从而证实经RPMI 1640琼脂培养形成的小菌落为毒力型菌落。人们发现该菌毒力株经组织培养后可分化变异成毒力型和无毒型菌落,这种变化与 This article describes a simple screening method for detecting toxic and non-toxic clones of Yersinia enterocolitica. The strain of virulent strains formed large and small colonies on RPMI 1640 agar (containing 40 mM HEPES and 1.5% pure agar). By self-agglutination test (a virulence marker) test, small colonies are often positive, while large colonies are negative. It was confirmed that the small colonies formed by RPMI 1640 agar culture were virulent colonies. It was found that the strain of virulent strains can be differentiated after tissue culture into virulent and non-toxic colonies, this change and