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目的:观察三七总皂苷对Nogo-A抑制神经细胞生长的拮抗作用。方法:选用人神经细胞系SHSY5Y细胞,采用Nogo-A(20×10-3μmol·L-1)对细胞产生抑制作用的方法,将细胞分为正常对照组,Nogo-A组,三七总皂苷大、小剂量组,Nogo-A+三七总皂苷大、小剂量组,维甲酸(retinoic acid,RA)组。用CCK-8法于加药后不同时间点检测细胞活力;并在倒置相差显微镜下观察加药后72 h的SH-SY5Y细胞的形态及轴突生长情况。结果:Nogo-A组对细胞活性及轴突生长产生明显抑制,胞体皱缩、变圆,突起减少,部分细胞漂浮于培养基中,而Nogo-A+三七总皂苷大、小剂量组细胞活性明显升高,细胞轴突伸长,仅有少量细胞胞体皱缩,细胞轮廓清晰,贴壁良好。且Nogo-A+三七总皂苷大剂量组细胞活性及轴突生长阳性细胞百分率高于Nogo-A+三七总皂苷小剂量组(P<0.05)。结论:三七总皂苷可以起到拮抗Nogo-A对神经细胞生长的抑制作用,促进轴突的生长,并存在一定的量效关系。
Objective: To observe the antisense effect of Panax notoginseng saponins on Nogo-A inhibit the growth of nerve cells. Methods: The human neural cell line SHSY5Y cells were selected and the cells were divided into normal control group, Nogo-A group, Panax notoginseng saponins by using Nogo-A (20 × 10-3μmol·L-1) Large and small dose group, Nogo-A + Panax notoginseng saponins large and small dose group, retinoic acid (RA) group. The viability of SH-SY5Y cells at 72 h after drug addition was observed under an inverted phase contrast microscope with CCK-8 method at different time points after drug addition. Results: Nogo-A group significantly inhibited cell viability and axon growth, cell body shrinkage, rounded, protrusions decreased, some cells floating in the medium, and Nogo-A + Panax notoginseng saponins large and small doses of cell activity Significantly elevated cell axons elongation, only a small amount of cell body shrinkage, cell outline clear, good adhesion. The percentages of cell viability and axon growth of Nogo-A + Panax Notoginseng Saponins were higher than that of Nogo-A + Panax Notoginseng Saponin (P <0.05). Conclusion: Panax notoginseng can antagonize the inhibitory effect of Nogo-A on the growth of nerve cells and promote the growth of axons, and there is a certain dose-effect relationship.