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以‘红地球’葡萄(Vitis vinifera L.‘Red Globe’)试管苗为试材,采用密闭系统落差法研究了在不同温度和光照强度下培养28d的试管苗的光合特性。结果表明:培养温度在20～30℃之间,试管苗的暗呼吸速率(Rd)随温度的升高而升高,但净光合速率(Pn)以25℃最高,30℃次之,20℃最低;而CO2补偿点以25℃最低,20℃次之,30℃最高;光照强度在40～200μmol·m-2·s-1之间,葡萄试管苗的Pn随光照强度(PAR)的升高而升高,CO2补偿点随PAR的升高而降低。在光照条件下,容器内CO2浓度迅速降低,并接近CO2补偿点,CO2供应不足是影响试管苗同化能力的主要原因。在室内培养阶段,采用弱光、昼夜变温和改善培养容器的通气性有利于提高试管苗的光合能力;在移栽驯化过程中,逐步提高光照强度和延长光照时间有利于试管苗同化产物的积累和培养壮苗。
Taking the test tube plantlets of ’Vitis vinifera L.’Red Globe’ as test material, the photosynthetic characteristics of in vitro plantlets cultured for 28 days under different temperature and light intensity were studied by using the closed system drop method. The results showed that the dark respiration rate (Rd) increased with the increase of temperature at the culture temperature of 20-30 ℃, but the highest Pn was at 25 ℃, followed by 30 ℃ and 20 ℃ While the CO2 compensation point was lowest at 25 ℃, followed by 20 ℃ and highest at 30 ℃, and the light intensity was between 40 and 200 μmol · m-2 · s-1. The Pn of grape seedlings increased with light intensity (PAR) High and increased, CO2 compensation point with the increase of PAR decreased. Under the light conditions, the CO2 concentration in the container decreased rapidly and close to the CO2 compensation point. The shortage of CO2 supply was the main reason that affected the assimilation ability of the plantlets. In the indoor cultivation stage, the use of weak light, temperature change day and night to improve the aeration of the culture container is conducive to improve the photosynthetic capacity of in vitro plantlets; in the transplanting and domestication process, gradually increase the light intensity and prolong the light time is conducive to the accumulation of plantlet assimilation products And cultivate strong seedlings.