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目的研究大鼠溃疡性结肠炎(UC)血、肠黏膜中一氧化氮(NO)和超氧化歧化酶(SOD)变化及丹参对其影响。方法予雌性Sprague-Dawley大鼠含30 mg三硝基苯磺酸(TNBS)的50%乙醇溶液0.85 ml灌肠,诱导实验性溃疡性结肠炎。造模7 d后,健康对照组、模型组尾静脉注入0.9%NaCl溶液1 ml/d;丹参治疗组每天尾静脉注入丹参注射液2 ml·kg~(-1)·d~(-1)。通过肠道重量指数、溃疡面积、大体形态和组织学评分及血和肠黏膜中NO、SOD含量测定评估疗效。结果丹参治疗组肠道重量指数、溃疡面积、大体形态和组织学评分及血和肠黏膜中NO含量减少、SOD含量增高,与模型组比较差异有统计学意义(P<0.01);但较健康对照组NO含量仍增高和SOD含量减少(P<0.05)。结论UC大鼠血、肠黏膜中NO和SOD异常,可能是UC发生和发展主要因素之一;丹参有效治疗UC与其拮抗NO、氧自由基机制有关。
Objective To study the changes of blood and intestinal mucosal nitric oxide (NO) and superoxide dismutase (SOD) in rats with ulcerative colitis (UC) and the effect of Danshen on it. Methods Experimental ulcerative colitis was induced in female Sprague-Dawley rats containing 0.85 ml of 50% ethanol solution of trinitrobenzene sulfonic acid (TNBS) 0.85 ml. After 7 days of modeling, 0.9% NaCl solution 1 ml / d was injected into the tail vein of healthy control group and model group. Salvia miltiorrhiza injection group was injected intravenously with Danshen injection 2 ml · kg ~ (-1) · d ~ (-1) . The curative effect was evaluated by intestinal mass index, ulcer area, gross morphology and histological score, and NO and SOD in blood and intestinal mucosa. Results Compared with the model group, the intestinal mucosa index, ulcer area, gross morphology and histological score of Salvia Miltiorrhiza in the treatment group decreased, while the content of NO in the blood and intestinal mucosa decreased and the content of SOD increased (P <0.01) The content of NO in the control group was still increased and the content of SOD was decreased (P <0.05). Conclusions The abnormality of NO and SOD in the blood and intestinal mucosa of UC rats may be one of the main factors for the occurrence and development of UC. The effective treatment of UC by Salvia miltiorrhiza is related to its antagonism of NO and oxygen free radical mechanism.