基于纳米生物共轭的酶活探针由于纳米材料的独特光电及电化学性质,多年来已经进行了广泛的研究。本综述总结了纳米金生物共轭探针在检测酶活性方面的研究成果,依次讨论了比色法、荧光法以及其他的方法,对其独特的设计进行了解释并讨论了各方法的优缺点。最后,简要指出了其发展的方向以及成功应用于实际应用时所存在的障碍。 Due to the unique photoelectric and electrochemical properties of nanomaterials, enzymatic probes based on nanobioconjugates have been extensively studied over the years. This review summarizes the research results of nano-gold bioconjugated probes in the detection of enzyme activity, discusses colorimetric methods, fluorescence methods and other methods in turn, explains their unique design and discusses the advantages and disadvantages of each method . Finally, it briefly points out the direction of its development and the obstacles in its successful application in practical application.