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目的应用基因工程技术,对日本血吸虫次黄嘌呤-鸟嘌呤磷酸核糖转移酶(hypoxanthine-guaninephosphoribosyltransferase,HGPRT)进行亚克隆,试图构建真核重组DNA质粒pcDNA3-HGPRT。方法通过筛选日本血吸虫成虫cDNA文库,克隆HGPRT基因片段,然后将目的片段亚克隆入真核表达载体pcDNA3。结果成功构建了真核重组DNA质粒pcDNA3-HGPRT,为进一步研究HGPRT的功能奠定了基础。
Objective To construct the eukaryotic recombinant plasmid pcDNA3-HGPRT by subcloning hypoxanthine-guanine phosphorylbosyltransferase (HGPRT) by using gene engineering technology. Methods The cDNA library of adult Schistosoma japonicum was cloned and the HGPRT gene fragment was cloned. The target fragment was subcloned into the eukaryotic expression vector pcDNA3. Results The eukaryotic recombinant plasmid pcDNA3-HGPRT was successfully constructed, which laid the foundation for further study on the function of HGPRT.