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目的:研究阿魏酸钠(SF)对人主动脉平滑肌(HASMC)和内皮细胞(HAEC)的影响,探讨SF成为抑制支架内再狭窄药物的机制。方法:HASMC和HAEC经SF处理后(0-1000μg/ml),用CCK-8试剂和划痕愈合试验检测不同药物浓度对两种生长和细胞迁移能力的影响;采用免疫细胞化学和Western blot检测HAECs中FoxM1和VEGF的表达。结果:SF对两种细胞的作用呈剂量依赖性,SF在10-1000μg/ml浓度时,HASMC的生长活力明显降低,在0.1-100μg/mlHAECs生长活力显著增强(P<0.05)。在1-1000μg/ml浓度下HASMCs迁移能力受到抑制,HAECs的迁移能力明显增加(0.1-100μg/ml)(P<0.05)。同时HAECs内FoxM1和VEGF表达明显增高(P<0.05),程度与SF浓度有剂量依赖关系。结论:阿魏酸钠能抑制血管平滑肌的增值和迁移;同时增加内皮细胞FoxM1和VEGF的表达,促进内皮细胞的增值和迁移,这些特征使其可能成为抑制支架内再狭窄的药物。
OBJECTIVE: To study the effect of sodium ferulate (SF) on human aortic smooth muscle (HASMC) and endothelial cells (HAEC), and to explore the mechanism of SF as an in-stent restenosis drug. Methods: HASMC and HAEC were treated with SF (0-1000μg / ml). The effects of different drug concentrations on the growth and cell migration were tested by CCK-8 reagent and scratch healing assay. Immunocytochemistry and Western blot Expression of FoxM1 and VEGF in HAECs. Results: The effect of SF on the two cell lines was dose-dependent. SF at 10-1000μg / ml significantly decreased the viability of HASMCs and increased the viability of HAECs at 0.1-100μg / ml (P <0.05). The migration ability of HASMCs was inhibited at the concentration of 1-1000μg / ml, and the migration ability of HAECs increased obviously (0.1-100μg / ml) (P <0.05). At the same time, the expression of FoxM1 and VEGF in HAECs were significantly increased (P <0.05), and the concentration of FoxM1 and VEGF in a dose-dependent manner. CONCLUSION: Sodium ferulate can inhibit the proliferation and migration of vascular smooth muscle cells, increase the expressions of FoxM1 and VEGF in endothelial cells, and promote the proliferation and migration of endothelial cells. These characteristics make it possible to be an agent for inhibiting in-stent restenosis.