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目的:探讨发酵罐酸洗钝化对百日咳杆菌生长和产毒的影响。方法:定期对百日咳发酵罐进行酸洗钝化处理，去除其表面附着的代谢螯合物。通过检测后续培养收获物菌浓度(550 nm吸光度值)、一次盐析沉淀量、发酵产物中百日咳毒素(pertussis toxin，PT)和丝状血凝素(filamentous haemagglutinin，FHA)含量，比较分析酸洗钝化对发酵培养和产毒的影响。结果:对处理前后各连续10批相关指标进行比较，处理后菌体550 nm吸光度均值由4.663提高至5.490，一次盐析沉淀量均值由4.84 g/L提高至5.90 g/L，PT平均含量达到3.3 μg/ml，FHA平均含量达到7.6 μg/ml，处理前后各连续10批菌体浓度和一次盐析沉淀量数据采用n t检验计算出n P值分别为0.03和0.05，差异均有统计学意义。n 结论:定期对发酵罐进行酸洗钝化可有效改善百日咳杆菌发酵及产毒水平。“,”Objective:To investigate the effects of fermenter pickling and passivation on the growth of n Bordetella pertussis and the toxin expression.n Methods:The metabolic chelates attached to the surface of fermenter were eliminated by pickling and passivation. Then the subsequent bacterial concentration of the cultivation, the weight of the first salting-out precipitation, the pertussis toxin (PT)/filamentous haemagglutinin (FHA) concentrations of the culture were detected to estimated the effect of the fermenter pickling and passivation.Results:Ten successive results showed that the mean value of bacterial concentration increased from 4.663 to 5.490, and the mean value of first salting-out precipitation increased from 4.84 g/L to 5.90 g/L. The average PT content reached 3.3 μg/ml and FHA reached 7.6 μg/ml, The data of 10 successive results of bacterial cell concentration and first salting-out precipitation were calculated by n t test with n P values of 0.03 and 0.05, respectively, and the differences in the data were statistically significant.n Conclusion:Fermenter pickling and passivation is an effective way to improve the growth of n Bordetella pertussis and the toxin expression.n