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目的探讨自行研制的微型植入式脊髓电刺激系统应用于动物实验的可行性。方法取健康成年SD大鼠20只,将微型植入式脊髓电刺激系统电极置于大鼠C2颈髓背侧硬膜外,脉冲发生装置及电源植入背部皮下。分别于术前(0 h)及术后12、24、36 h,测试微型植入式脊髓电刺激系统的参数(电压、刺激频率和脉宽);进行大鼠神经行为学功能评分(BBB评分)和斜板实验,并测量大鼠体质量。结果脊髓电刺激系统各项参数在0 h、12 h、24 h时点间比较,差异均无统计学意义(P>0.05);各项参数在0 h与36 h时点间比较,差异有统计学意义(P<0.05)。BBB评分、斜板实验和体质量检测显示:0 h与12 h时点比较,差异有统计学意义(P<0.05);0 h与24、36 h时点比较,差异无统计学意义(P>0.05)。实验过程中大鼠无明显不良反应。结论微型植入式脊髓电刺激系统应用于动物实验是安全可行的,可用于脊髓电刺激治疗脑缺血性疾病的进一步实验研究。
Objective To investigate the feasibility of using a miniature implantable spinal cord electrical stimulation system developed by ourselves in animal experiments. Methods Twenty healthy adult Sprague-Dawley rats were implanted with miniature implantable spinal cord electrostimulation system electrodes in the dorsal epidural space of C2 cervical spinal cord of rats. The pulse generator and the power supply were implanted subcutaneously in the back. The parameters (voltage, stimulation frequency and pulse width) of the mini-implanted spinal electrical stimulation system were measured before operation (0 h) and at 12, 24 and 36 h after operation respectively. The neurobehavioral function score (BBB score ) And inclined plate experiment, and measure the body weight of rats. Results There was no significant difference in the parameters of spinal cord electrical stimulation system between 0 h, 12 h and 24 h (P> 0.05). The differences of parameters between 0 h and 36 h Statistical significance (P <0.05). The score of BBB, the experiment of ramp and the test of body weight showed that the difference was statistically significant at 0 h and 12 h (P <0.05), and there was no significant difference at 0 h and 24 h and 36 h > 0.05). There was no obvious adverse reaction in rats during the experiment. Conclusion The micro-implantable spinal cord electrical stimulation system is safe and feasible for animal experiments and can be used for further experimental study of spinal cord stimulation in the treatment of cerebral ischemic disease.