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为寻求猪丹毒菌种经保存后其活力、毒力和免疫原性不受影响的保存方法,以供保存制造猪丹毒生物制品和检验用的菌种,我们自1953年至1957年曾采用冻干,继代,密封,组织材料冻干和半固体等五种方法进行了猪丹毒菌种保存试验工作,兹将其经过及结果叙述如下,供读者参考。猪丹毒菌种的保存方法猪丹毒菌种冻干保存方法:将经过细菌学鉴定的猪丹毒杆菌接种在10％血清(马或牛)马丁琼脂斜面上,于37℃
To ensure that the virulent, virulence and immunogenicity of pig erysipelas strains are not affected after their storage, for the preservation of the production of erysipelas bioproducts and strains for testing, we have used frozen insects from 1953 to 1957. Dry, sub-generation, sealing, freeze-dried and semi-solid tissue materials were used in five methods for the preservation test of erysipelas, and their results were described below for reference of the reader. Method for the preservation of erysipelas porcine strains. Method for lyophilization of erysipelas porvivia strains: Bacteriologically identified erysipelas is inoculated on a 10% serum (horse or bovine) Martin agar slant at 37°C.