论文部分内容阅读
本文研究人参总皂苷在正常和炎症状态下对HepG2细胞CYP2B6 mRNA和蛋白表达的影响。采用索式提取法提取人参总皂苷,Real-Time PCR及Western Blot技术检测细胞中CYP2B6 mRNA和蛋白表达。结果显示,正常状态下人参总皂苷低(15 g/mL)、中(60 g/mL)与高(240 g/mL)剂量均显著诱导CYP2B6 mRNA表达(p<0.05),中、高剂量均显著诱导CYP2B6蛋白的表达(p<0.05)。使用LPS孵育细胞制造炎症模型,LPS对CYP2B6 mRNA表达无影响,但显著诱导CYP2B6蛋白的表达(p<0.05)。使用人参总皂苷和LPS共同孵育细胞后,炎症状态下人参总皂苷中、高剂量对CYP2B6 mRNA的表达仍有显著诱导效果(p<0.05),低和中剂量则均能显著诱导CYP2B6蛋白的表达,但高剂量则对CYP2B6的蛋白表达则呈现抑制作用(p<0.05)。提示炎症状态能够改变人参总皂苷对CYP2B6 mRNA和蛋白的表达,因此联合用药时,应考虑不同生理状态下人参对代谢性药物相互作用的影响。
This study was to investigate the effects of ginsenosides on the expression of CYP2B6 mRNA and protein in HepG2 cells under normal and inflammatory conditions. Total ginsenosides were extracted by Soxhlet extraction. The expression of CYP2B6 mRNA and protein was detected by Real-Time PCR and Western Blot. The results showed that total ginsenoside (15 g / mL), medium (60 g / mL) and high (240 g / mL) significantly induced the expression of CYP2B6 mRNA (p <0.05) Significant induction of CYP2B6 protein expression (p <0.05). LPS did not affect CYP2B6 mRNA expression, but significantly induced the expression of CYP2B6 protein (p <0.05) using LPS to incubate cells to create an inflammatory model. After incubated with total ginsenosides and LPS, CYP2B6 mRNA expression was still significantly up-regulated (P <0.05) at both high and low doses of total ginsenoside in the inflammatory state, while both CYP2B6 and CYP2B6 were significantly induced by both low and medium doses , But the high dose of CYP2B6 protein expression was inhibited (p <0.05). Suggesting that inflammatory state can change the expression of total ginsenoside CYP2B6 mRNA and protein, so the combination of drugs, should take into account the different physiological state of ginseng on metabolic drug interactions.