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目的 研究白介素 17(IL 17)在溃疡性结肠炎 (UC)的表达和分泌及与疾病活动性的关系。方法 用ELISA法测定UC患者及正常对照者血清或细胞培养液中 ,IL 17、IL 6和IL 8的浓度 ,逆转录聚合酶链反应 (RT PCT)测定IL 17mRNA的表达。结果 32例UC患者外周血中IL 17,IL 6和IL 8的浓度与 40例正常对照者比较 ,差异无显著性 (P >0 .0 5 ) ,但外周血CD+4T细胞在PMA和抗CD3 的刺激下 ,表达IL 17mRNA及分泌IL 17的水平均明显高于对照组 [(2 3.6± 5 .7) pg/ml和 (13.1± 3.2 ) pg/ml,P <0 .0 1]。UC患者病变部位的黏膜固有层CD+4T细胞 (LP CD+4T)与非受累部位的LP CD+4T细胞比较 ,它们表达大量的IL 17mRNA并自发分泌大量的IL 17蛋白 ,且IL 17浓度与该部位的单个核细胞 (LPMC)分泌的IL 6 ,以及患者外周血中的C 反应蛋白 ,血沉均呈显著正相关。在刺激剂的作用下 ,病变部位的LP CD+4T细胞IL 17的分泌进一步增加 ,且明显高于非受累部位LP CD+4T细胞的分泌水平。另外 ,UC病变部位LPMC分泌的IL 6和IL 8的水平均明显高于非受累部位的LPMC ,但在培养液中加入抗IL 17单克隆抗体后 ,LPMC细胞IL 6和IL 8的分泌均明显被抑制。结论 UC患者病变部位的LP CD+4T细胞表达和分泌IL 17明显增加 ,并与疾病的活动性呈正相关。抗IL 17抗
Objective To study the expression and secretion of interleukin 17 (IL 17) in ulcerative colitis (UC) and its relationship with disease activity. Methods The concentrations of IL-17, IL-6 and IL-8 in serum or cell culture medium of UC patients and normal controls were measured by ELISA. The expression of IL-17 mRNA was measured by reverse transcriptase-polymerase chain reaction (RT-PCR) Results The concentrations of IL 17, IL 6 and IL 8 in peripheral blood of 32 UC patients were not significantly different from those of 40 normal controls (P> 0.05), but the levels of PMA and anti-CD The levels of IL-17 mRNA and IL-17 secreted by CD3 were significantly higher than that of the control group [(3.6 ± 5.7) pg / ml and (13.1 ± 3.2) pg / ml, P <0.01). Compared with LP CD + 4T cells in non-affected sites, lamina propria CD + 4T cells (LP CD + 4T) in diseased parts of UC patients expressed a large amount of IL 17 mRNA and secrete a large amount of IL 17 protein spontaneously, and IL 17 concentration and There was a significant positive correlation between IL-6 secreted by LPMC and C-reactive protein and erythrocyte sedimentation rate in peripheral blood of the patients. The secretion of IL-17 by LPCD + 4T cells at the lesion site was further increased by the stimulant, and was significantly higher than that at the LP CD + 4T cells at the non-affected site. In addition, the levels of IL-6 and IL-8 secreted by LPMC in UC lesions were significantly higher than those in non-affected sites, but the secretion of IL-6 and IL-8 in LPMC was significantly increased after anti-IL-17 monoclonal antibody suppressed. Conclusion The expression and secretion of IL-17 in LP CD + 4T cells in the diseased parts of UC patients were significantly increased, and positively correlated with the activity of the disease. Anti-IL 17 Antibody