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报道了将ＤｒＭａｒｃＺａｂｅａｕ的ＡＦＬＰ（ＡｍｐｌｉｆｉｅｄＦｒａｇｍｅｎｔＬｅｎｇｔｈＰｏｌｙｍｏｒ－ｐｈｉｓｍ）技术中的ＤＮＡ提取方法用于以ＰＣＲ（ＰｏｌｙｍｅｒａｓｅＣｈａｉｎＲｅａｃｔｉｏｎ）技术鉴定转基因植物——拟南芥、烟草和芜菁中。该方法需约１ｈ可完成植物ＤＮＡ的提取，样品用量仅为３０～１００ｍｇ，产量可达３０～８０μｇ／ｇ．粗提ＤＮＡ（溶于１０μＬＴＥ缓冲液）不必经过纯化，用ＴＥ缓冲液稀释５～１０倍即可直接用于ＰＣＲ分析。在拟南芥、烟草和芜菁转基因植物鉴定中应用表明这一方法是目前以ＰＣＲ法鉴定转基因植株Ｔ１代单株幼苗的一种简便、快速、有效的方法。
Reported that the DNA extraction method in the AFLP (Amplified Cell Growth Polymor-phism) technology by Dr Marzabeau was used to identify transgenic plants - Arabidopsis thaliana, tobacco and turnip with PCR (Polymerase Chain Reaction) technology. The method takes about 1h to complete the extraction of plant DNA, the sample dosage is only 30 ~ 100mg, the yield can reach 30 ~ 80μg / g. Crude DNA (dissolved in 10 μL TE buffer) without purification, diluted 5 to 10 times with TE buffer can be used directly for PCR analysis. The application of the method in the identification of transgenic Arabidopsis, tobacco and turnip transgenic plants shows that this method is a simple, rapid and effective method for the identification of T1 seedlings of transgenic plants by PCR.