论文部分内容阅读
目的 :研究氰戊菊酯 (Fen)对雄性生殖内分泌系统的损害作用及其机制。 方法 :将不同剂量的Fen(0、2 .4、12、6 0mg/kg) ,每日分别对雄性成年SD大鼠连续灌胃 ,染毒 15、30d ,应用RIA法测定大鼠血清中FSH、LH、T和睾丸匀浆中T的水平 ,同步测定睾丸标志酶ACP、γ GT的活性 ,并采用精子头计数法观测每日精子生成量 (Spr)的变化。 结果 :与对照组相比 ,染毒 15d时 ,血清中FSH水平在≤ 12mg/kg剂量组均明显升高 (P <0 .0 1) ,血清中LH含量在 12mg/kg剂量组显著增加 (P <0 .0 1) ,而睾丸匀浆中T在≥ 12mg/kg剂量组中表现为显著下降 (P<0 .0 1) ;染毒至 30d时 ,血清中FSH水平在≥ 12mg/kg剂量范围继续呈现显著增加 (P <0 .0 1) ,睾丸匀浆中T在2 .4mg/kg剂量组则降低 (P <0 .0 5 )。ACP活性在染毒 15d时 2 .4mg/kg剂量组表现为升高 (P <0 .0 5 ) ,继续染毒至 30d时 6 0mg/kg剂量组则显著减低 (P <0 .0 5 ) ;γ GT活性则始终随染毒剂量的增加而降低 (P <0 .0 5 )。Spr与染毒剂量有明显的剂量依赖关系 ,在≥ 12mg/kg剂量范围显著减少 (P <0 .0 1)。 结论 :Fen对雄性大鼠有明显的生殖毒性 ,可影响其血清及睾丸性激素水平和酶活性 ,这可能与Fen对支持细胞和生精上皮的损害有关
Objective: To study the damaging effect of fenvalerate on male reproductive endocrine system and its mechanism. Methods: Adult male Sprague-Dawley rats were fed with Fen (0,2. 4,12,6 0 mg / kg) at different doses for 15 and 30 days. RIA was used to determine the serum levels of FSH , LH, T and testicular homogenate. The activities of testis enzymes ACP and γ GT were measured synchronously. The changes of daily sperm production (Spr) were observed by sperm counting method. Results: Compared with the control group, the level of FSH in the serum was significantly increased (P <0.01) in the group of ≤ 12 mg / kg at 15 d and the level of LH in serum was significantly increased at 12 mg / kg P <0.01), while T in testis homogenates showed a significant decrease (P <0.01) in the group of ≥ 12 mg / kg dose. Serum levels of FSH were higher than 12 mg / kg The dose range continued to show a significant increase (P <0. 01), testicular homogenate T in the 2.4 mg / kg dose group was reduced (P <0. The activity of ACP increased at the dose of 2.4 mg / kg (P <0.05) on the 15th day of exposure, and decreased significantly at the dose of 60 mg / kg at 30 days (P <0.05) ; γ GT activity was always decreased with the increase of dose (P <0.05). There was a clear dose-dependent relationship between Spr and exposure dose, with a significant reduction at doses ≥ 12 mg / kg (P <0.01). Conclusion: Fen has obvious reproductive toxicity in male rats, which may affect the serum and testicular hormone levels and enzyme activity, which may be related to the damage of Fen on the supporting cells and the seminiferous epithelium