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目的:探讨氯胺酮不同给药方法对脓毒症大鼠血清肿瘤坏死因子-α(TNF-α)的影响。方法:选择健康雄性SD大鼠24只,随机分为四组:对照组、CLP(盲肠结扎加穿孔)组、氯胺酮(KT)组和KT组。对照组开腹后不做任何处理,其余各组均行CLP,CLP后开始计时。KT组于术前30 min,KT组于CLP后2 h开始,均经尾静脉给予氯胺酮5 mg.kg-1.h-1至CLP5 h,术毕用酶联免疫吸附试验检测血清TNF-α。结果:对照组、KT、KT组血清TNF-α均低于CLP组(P<0.05),KT与KT组比较差异有显著性(P<0.05)。结论:氯胺酮不同给药方法对脓毒症大鼠均有保护效应,术前输注效果更佳。
Objective: To investigate the effects of different administration of ketamine on serum tumor necrosis factor-α (TNF-α) in septic rats. Methods: Twenty-four healthy male Sprague-Dawley rats were randomly divided into four groups: control group, CLP group (cecal ligation and perforation), ketamine (KT) group and KT group. The control group did not do any treatment after laparotomy, the remaining groups were CLP, CLP began timing. Thirty minutes before operation in KT group, KT group started at 2 hours after CLP, and ketamine 5 mg.kg-1.h-1 to CLP 5 h were administered through the tail vein. Serum TNF-α was detected by enzyme-linked immunosorbent assay . Results: The levels of TNF-α in KT and KT groups were lower than those in CLP group (P <0.05). There was significant difference between KT group and KT group (P <0.05). Conclusion: Different administration of ketamine has protective effect on septic rats, preoperative infusion better.