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本文建立了利多卡因血药浓度的非均相竞争抑制酶免疫分析方法。每次测定需全血样品100μ1,在2-3小时内完成。用生物素-抗生物素系统将辣根过氧化物酶(HRP)标记利多卡因专一性抗体,对利多卡因测定灵敏(0.25μg/ml或0.05μg/孔),专一,与利多卡因的衍生物室安卡因及其它抗心律失常药物无交叉反应。其变异系数(CV)批内为9.8-19.7%。批间为4.3-16.8%,全血标准曲线的相关系数(r)为0.992。可用于对临床病人利多卡因血药浓度的监测,方法简便,实用。
This article established a lidocaine plasma concentration heterogeneous competitive inhibition enzyme immunoassay. Each assay requires 100 μl of whole blood sample and is completed within 2-3 hours. Horseradish peroxidase (HRP) -labeled lidocaine-specific antibodies were biotin-avidin-sensitive and sensitive to lidocaine (0.25 μg / ml or 0.05 μg / well), specific, There was no cross-reaction of the Cocaine derivative, Room Ankaine, and other anti-arrhythmic drugs. The coefficient of variation (CV) within the batch is 9.8-19.7%. 4.3-16.8% between batches, and the correlation coefficient (r) for the whole blood standard curve was 0.992. It can be used to monitor the concentration of lidocaine in clinical patients. The method is simple and practical.