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国内、外对胰岛细胞的特殊染色法不断得到了改进和完善。这些方法对A细胞和B细胞比较稳定,但D细胞染色仍不够理想。本法是在Gomori醛复红基础上,根据胰岛各种细胞分泌颗粒的性质,对酸碱性染料亲和能力的特性,选用了醛复红染B细胞,橘黄G丽春红G染A细胞,苯胺蓝染D细胞。我们用此法进行了反复试验,稳定可靠,效果良好,适合制作多量的教学切片标本。方法如下:一、取豚鼠新鲜胰腺,Helly’s液固定24小时,中
Domestic and foreign islet cells of the special staining method has been continuously improved and improved. These methods are relatively stable on A cells and B cells, but D cell staining is still not ideal. This method is based on the Gomori aldehyde complex red, based on the nature of the various cells of pancreatic cell secretory particles, the affinity of the acid-base dye characteristics, the selection of aldehyde complex red stained B cells, orange G Li Chunhong G stained A cells , Aniline blue stained D cells. We use this method has been repeated testing, stable and reliable, good effect, suitable for the production of large quantities of teaching specimens. Method is as follows: First, take guinea pigs fresh pancreas, Helly’s fluid fixed 24 hours, in