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目的:探索口服携带人PF4基因的减毒沙门氏菌对大剂量化疗小鼠造血重建作用。方法:通过在大剂量化疗前／后喂服携带PIRES2-EGFP／PF4减毒沙门氏菌,检测化疗后小鼠的生存率,小鼠在不同时间外周血血常规、骨髓细胞数、骨髓中Sca-1和C-kit细胞含量、不同时间骨髓生成各系细胞的集落数等。结果:在大剂量化疗前、后均口服携带PIRES2-EGFP／PF4减毒沙门氏菌组小鼠生存率高于只在化疗前口服携带PIRES2-EGFP／PF4减毒沙门氏菌组;两组口服携带PIRES2-EGFP／PF4减毒沙门氏菌组小鼠存活率明显高于PBS对照组及携带空载体的减毒沙门氏菌组;口服携带PIRES2-EGFP／PF4减毒沙门氏菌组小鼠在化疗后第9~12天的外周血血小板数、骨髓细胞中Sca-1和C-kit阳性细胞含量明显比对照组高,第5天、9天、12天的骨髓细胞总数、骨髓细胞形成Mix集落数明显增加。结论:口服减毒沙门氏菌SL3261为载体的PF4基因可以保护小鼠免受损伤,并促进化疗损伤小鼠的造血恢复。
Objective: To explore the role of attenuated Salmonella typhimurium oral PF4 gene in hematopoietic reconstitution in high-dose chemotherapy mice. Methods: Salmonella typhimurium was inoculated with PIRES2-EGFP / PF4 before and after high-dose chemotherapy. The survival rate of mice after chemotherapy was measured. The blood levels of blood, bone marrow cells, bone marrow Sca-1 And C-kit cell content, bone marrow cells at different times to generate the number of colonies and other cells. Results: The survival rate of mice in PIRES2-EGFP / PF4-attenuated Salmonella group orally before and after high-dose chemotherapy was higher than that of PIRES2-EGFP / PF4-attenuated salmonella group only orally before chemotherapy; the two groups orally carried PIRES2-EGFP / PF4 attenuated Salmonella group was significantly higher than that of the PBS control group and the attenuated Salmonella group carrying the empty vector; the oral administration of the PIRES2-EGFP / PF4 attenuated Salmonella group mice in the peripheral blood of 9 to 12 days after chemotherapy Platelet count, Sca-1 and C-kit positive cells in bone marrow cells significantly higher than the control group, the first 5 days, 9 days, 12 days the total number of bone marrow cells, bone marrow cells to form a significant increase in the number of Mix colonies. Conclusion: The PF4 gene of oral attenuated Salmonella SL3261 can protect mice from injury and promote hematopoietic recovery in mice with chemotherapy-induced injury.