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Monitoring expression at the transcriptional level is the first essential step for the functional analysis of plant genes.Genes-encoding proteins directly involved in early response to elicitor constitute only a small fraction of all the genes affected by elicitor. TranscriptiOnal responses to various elicitors have been extensively studied in different plants including Nicotiana and Arabidopsis thaliana;however,corresponding data arent available for non-heading Chinese cabbage.To address this problem,we describe a suppression subtractive library-based approach to isolate the plants ESTs up-regulated in the early induction/execution of the HR induced by elicitor PB90 from Phytophthora boehmeriae. According to their putative identification in BLAST searches against the three genome databases,70 up-regulated genes were classified into 9 parts:some aspect of primarymetabolismorenergyproduction;protein synthesisorprotein fate;cellular communication/signal transduction mechanism;cell fates including Beclin,SPT1,and SPT2;HLA-B and AGO1 which participate in transcription;cellular transport and hypothetical proteins or proteins for which a function has yet to be determined.Seven selected genes such as Beclin,thioredoxin,HLA-B,MAP3K,SPT1,SPT2,and AGO1 were up-regulated induced by PB90,suggesting that the genes may play an important role in PB90-triggered HR.