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目的研究去甲斑螯素所诱导的蛋白磷酸酶2A(PP2A)的抑制对鼻咽癌细胞系CNE1及其裸鼠移植瘤放射增敏的影响。方法采用免疫共沉淀、流式细胞术等方法探讨去甲斑螯素(40μmol·L~(-1))或(和)放射处理(8 Gy)对鼻咽癌细胞CNE1及其裸鼠移植瘤PP2A活性、细胞周期、凋亡的影响情况,研究去甲斑螯素抑制PP2A后于放射作用下所诱导的鼻咽癌细胞CNE1及其移植瘤组织生物学现象的变化。结果去甲斑螯素协同放射处理相比较其他各处理组,G2/M期细胞显著聚集增多(45.71±2.015)%,凋亡率也明显提高(75.63±6.11)%,裸鼠移植瘤抑瘤率达到87.64%。结论 PP2 A的抑制显著诱导鼻炎癌细胞的凋亡与延迟移植瘤的生长,其很有可能成为提高鼻咽癌放射增敏作用的新靶点。
Objective To investigate the effect of nigrastatin - induced inhibition of protein phosphatase 2A (PP2A) on radiosensitization of nasopharyngeal carcinoma cell line CNE1 and its nude mice xenografts. Methods Immunoprecipitation, flow cytometry and other methods were used to investigate the effects of nystatin (40μmol·L -1) or (8g) irradiation on nasopharyngeal carcinoma cells CNE1 and its nude mice xenografts PP2A activity, cell cycle and apoptosis of nasopharyngeal carcinoma cell line CNE1 and its xenograft tumor induced by radiation. Results Compared with other treatment groups, the synergistic radiation treatment of norcantharidin significantly increased the number of G2 / M phase cells (45.71 ± 2.015)% and the apoptotic rate significantly (75.63 ± 6.11)% Rate reached 87.64%. Conclusion PP2 A can significantly induce the apoptosis of nasopharyngeal carcinoma cells and delay the growth of xenografts. It is likely to be a new target of enhancing radiosensitization of nasopharyngeal carcinoma.