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目的:探讨Wnt/β-catenin基因和多发性骨髓瘤骨病的关系。方法:分离培养多发性骨髓瘤患者和正常人的骨髓间充质干细胞(MSCs),体外诱导分化成骨细胞,茜素红实验检测矿化物沉积、荧光定量RT-PCR方法检成骨指标(OPN、OC、ALP、Cbfal)和Wnt/β-catenin mRNA表达,分析MSCs细胞成骨能力变化及两组间Wnt/β-catenin mRNA表达差异。结果:骨髓MSCs体外成骨诱导后,茜素红染色阳性,呈现明显红色钙化结节;MSCs体外诱导成骨细胞,试验组与对照组比较,成骨指标OPN、OC、ALP、Cbfαl mRNA表达降低(P<0.05);骨髓MSCs体外成骨诱导后β-catenin mRNA表达降低(P<0.05)。结论:骨髓MSCs体外可成功诱导分化为成骨细胞;多发性骨髓瘤患者MSCs向成骨细胞分化潜能比正常人降低,Wnt/β-catenin可作为多发性骨髓瘤骨病潜在治疗靶点。
Objective: To investigate the relationship between Wnt / β-catenin gene and multiple myeloma. Methods: Bone marrow mesenchymal stem cells (MSCs) from patients with multiple myeloma and normal controls were isolated and cultured. Differentiated into osteoblasts in vitro. Alizarin red assay was used to detect the mineralization. OPN , OC, ALP, Cbfal) and Wnt / β-catenin mRNA expression were analyzed. The osteogenic potential of MSCs was analyzed and the differences of Wnt / β-catenin mRNA expression between the two groups were analyzed. Results: After osteogenic induction of bone marrow MSCs, alizarin red staining showed obvious red calcified nodules; osteoblasts were induced by MSCs in vitro. Compared with the control group, osteogenic markers OPN, OC, ALP and Cbfαl mRNA expression were decreased (P <0.05). The mRNA expression of β-catenin decreased after osteogenic induction of bone marrow MSCs (P <0.05). CONCLUSION: MSCs differentiate into osteoblasts in vitro successfully. MSCs differentiate into osteoblasts in multiple myeloma patients. The potential of Wnt / β-catenin as a potential therapeutic target for multiple myeloma is lower than that of normal human.