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目的探讨5-氮胞苷局部注入猪急性心肌梗死(AMI)梗死区后对心功能的修复作用。方法幼猪20只,开胸结扎冠状动脉前降支建立猪AMI模型;3周后治疗组(10只)通过局部注射将1mg/ml的5-氮胞苷溶液1ml注入梗死区及其周边;对照组(10只)予以等量的培养液接种。术前、术后3周及7周分别行MPA心功能测定;7周后处死所有动物,获取心脏标本,2,3,5-氯化三苯基四氮唑(TTC)染色测定心肌梗死的面积。结果TTC染色治疗组梗死区面积及所占百分比明显小于对照组(P<0.01)。与治疗组3周及对照组7周比较,治疗组7周的左室最高压、dP/dtmax对应的左室压和左室压上升最大变化速率(dP/dtmax)明显增高(P<0.01);左室压最低值、左室舒张末压明显降低(P<0.01);-dP/dtmax对应的左室压和左室压下降最大变化速率(-dP/dtmax)绝对值明显增高(P<0.01)。结论5-氮胞苷局部注入猪AMI梗死区后可以缩小心肌梗死面积,改善心脏的收缩和舒张功能。
Objective To investigate the repair effect of 5-azacytidine on cardiac function after infusing AMI infarction area locally. Methods Twenty newborn piglets were enrolled in this study. AMI model was established by thoracotomy and ligation of the anterior descending coronary artery. After 3 weeks, 10 ml of 5 mg / ml 5-azacytidine solution was infused into the infarct area and peri-infarct area by local injection. The control group (10 mice) was inoculated with the same amount of culture solution. MPA cardiac function was measured preoperatively, 3 weeks and 7 weeks after operation. All animals were sacrificed 7 weeks later, and heart samples were obtained. TTC staining was used to determine myocardial infarction area. Results The area and the percentage of infarction area in TTC staining group were significantly less than those in control group (P <0.01). Compared with 3 weeks in the treatment group and 7 weeks in the control group, left ventricular peak pressure and dP / dtmax corresponding to dP / dtmax and dP / dtmax increased significantly (P <0.01) (P <0.01). The absolute values of -dP / dtmax and -dP / dtmax corresponding to dP / dtmax were significantly higher (P < 0.01). Conclusion Local injection of 5-azacytidine into AMI infarct area can reduce myocardial infarct size and improve cardiac contractility and diastolic function.