作者对人类肿瘤性渗出液中有丝分裂细胞的染色体组型变化的研究,认为可能有助于诊断的确立,因为在静止期中,无论是间皮细胞抑或是肿瘤细胞都是形态变化多端,要鉴别它们是有困难的。在研究的过程中,作者还采用了一种改进的方法,具体操作步骤如下:将溶出液收集于含有肝素的离心管中,在37℃中,用水稀释5倍。保温20分钟后,离心10分钟(约1200转/分)。弃去上清液,用3份纯木醇和1份冰醋酸的新鲜冷混合液固定细胞沉淀,加固定液时切勿摇动沉淀物。在4℃保持1小时后离心,弃去上清液,再加固定液并振荡管子一段时间。用 Pasteur 氏 The authors’ study on the karyotype of mitotic cells in human tumoral exudate suggests that it may be helpful for the establishment of diagnosis, because in the quiescent period, whether the mesothelial cells or the tumor cells are morphological changes, we must identify They are difficult. During the course of the study, the authors also used an improved method. The specific procedure was as follows: The eluate was collected in heparin-containing centrifuge tubes and diluted 5 times with water at 37°C. After incubation for 20 minutes, centrifuge for 10 minutes (about 1200 rpm). Discard the supernatant and fix the cell pellet with a fresh mixture of 3 parts pure alcohol and 1 part glacial acetic acid. Do not shake the pellet when adding the fixative solution. After centrifugation at 4°C for 1 hour, discard the supernatant, add a fixative and shake the tube for a while. Pasteur’s