【摘 要】
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Advances in genome editing technology have revolutionized basic and applied biology research in recent years,particularly due to the newly emerged CRISPR/Cas te
【机 构】
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State Key Laboratory of Cell Biology, Shanghai Key Laboratory of Molecular Andrology, CAS Center for
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Advances in genome editing technology have revolutionized basic and applied biology research in recent years,particularly due to the newly emerged CRISPR/Cas technique (Ren et al.,2017b).The classical CRISPR/Cas system was derived from a bacterial defense system,which consists of a single guide RNA (sgRNA) for precise targeting and one Cas protein for DNA binding and nuclease activity.Relying on high precision of CRISPR/Cas system,multiple functions have been exploited in addition to original nuclease activity,such as genome base editing,gene knock-down and activation,chromatin imaging systems etc.
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