目的建立适合我国的幽门螺杆菌（Ｈｐ）感染相关性胃炎的猪模型．方法用中国１号小型猪为实验动物，剖腹取胎后，分为低酸（ｎ＝４）、高酸（ｎ＝４）和粘膜损伤（ｎ＝５）３组，经口感染Ｈｐ菌后，分别置于一、二级环境中饲养．用ＨＥ染色、ＷａｒｔｈｉｎＳｔａｒｒｙ银染色、尿素酶、ＨｐＰＣＲ及细菌培养等方法鉴定感染Ｈｐ是否造成Ｈｐ相关性胃炎的动物模型．结果粘膜损伤组不论在一级还是在二级环境中饲养的乳猪，其食管、胃、十二指肠的粘膜表面均可见Ｈｐ菌；尿素酶、ＨｐＰＣＲ检查均呈阳性反应；有３只乳猪培养出Ｈｐ菌；ＨＥ染色组织切片证实实验乳猪患有急、慢性胃炎．而低酸组、高酸组虽然尿毒酶检查，ＨｐＰＣＲ检查大多数呈阳性反应，但组织切片及培养均未见Ｈｐ．结论首次用中国１号小型猪成功地造成Ｈｐ感染的动物模型；胃粘膜呈急、慢性炎性改变．剖腹取胎无特殊病原体的乳猪用消炎痛损伤粘膜，为成功建立Ｈｐ感染胃炎提供良好的条件．在二级饲养下胃粘膜表面的Ｈｐ量明显多于一级饲养． Objective To establish a porcine model of H.pylori infection related gastritis in China. Methods The Chinese mini-pig No. 1 was used as experimental animals. The rats were divided into 3 groups: low acid (n = 4), high acid (n = 4) and mucosal injury (n = 5) , Were placed in one or two environment feeding. Animal models of Hp-associated gastritis were established by HE staining, Warthin-Starry silver staining, urease, Hp-PCR and bacterial culture. Results In the mucosal injury group, Hp bacteria were found on the mucosal surfaces of the esophagus, stomach and duodenum of piglets reared in primary or secondary environment; urease and HpPCR showed positive reaction; 3 Only piglets cultured Hp bacteria; HE staining tissue sections confirmed experimental suckling pigs with acute and chronic gastritis. The low acid group, high acid group although uremic enzyme test, Hp PCR most of the positive reaction, but the tissue sections and culture did not see Hp. Conclusions The animal model of Hp infection was successfully established in China No.1 pig for the first time. Gastric mucosa showed acute and chronic inflammatory changes. Caesarean section fetuses without special pathogens in pigs with indomethacin injury mucosa, in order to successfully establish gastritis Hp infection provide good conditions. The level of Hp on the surface of gastric mucosa was significantly higher than that of the first level in the second level feeding.