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目的和方法：利用人手术切除胃标本，应用Ｐｒｏｎａｓｅ－ＥＤＴＡ法进行人正常胃粘膜细胞的分离及孵育，并采用羟自由基建立细胞损伤模型，观察八肽生长抑素（ＳＳ－８）对细胞损伤的影响。结果：（１）采用Ｐｒｏｎａｓｅ－ＥＤＴＡ法分离人胃粘膜上皮细胞，每４ｃｍ×５ｃｍ大小胃粘膜可分离细胞数为２４×１０６个，细胞存活率达９０％以上。（２）Ｆｅ２＋－Ｈ２Ｏ２体系产生的羟自由基（ＯＨ·）可直接导致人胃粘膜细胞存活率下降，细胞乳酸脱氢酶漏出量及细胞丙二醛含量增加，以ＳＳ－８预处理可减轻上述改变。结论：ＳＳ－８可减轻ＯＨ·对孵育状态下人胃粘膜细胞的损伤，作用与其减轻细胞的脂质过氧化程度有关。
OBJECTIVE: To investigate the effect of SS-8 on the proliferation of human gastric mucosa cells and to explore the possible mechanism of gastric mucosal injury.Methods: The impact of injury. Results: (1) Human gastric mucosal epithelial cells were isolated by Pronase-EDTA method. The number of detachable cells per 4cm × 5cm gastric mucosa was 24 × 106, and the cell survival rate was over 90%. (2) Hydroxyl radical (OH ·) produced by Fe2 + -H2O2 system can directly lead to the decrease of cell viability of human gastric mucosa, cell leakage of lactate dehydrogenase and increase of malondialdehyde (MDA) The above change. Conclusion: SS-8 can reduce the OH · on human gastric mucosal injury under incubation, and its role in reducing cell lipid peroxidation level.