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目的探讨模拟微重力环境对记忆功能和学习相关蛋白表达的影响。方法研究采用7 d尾吊模拟微重力环境,利用水迷宫和跳台检测记忆能力,利用免疫印迹技术检测记忆相关蛋白、神经保护蛋白和抗氧化酶蛋白表达,利用RT-qPCR检测基因表达。结果 SD大鼠尾吊7 d后,空间记忆能力和位置记忆能力均减弱。尾吊降低了大鼠海马学习相关蛋白NR1/2B-CaMKⅡ-CREB通路的活化,机械生长因子MGF及其下游Nrf2介导的神经保护蛋白HO-1表达也明显减少。另外,尾吊抑制了抗氧化酶SOD2和GPx的基因和蛋白表达。这些结果提示,模拟微重力环境明显抑制了海马NR1/2B和MGF信号途径。结论 NR和MGF可能分别作为尾吊介导的认知障碍的调节因子和保护因子,这为微重力环境下认知障碍的学习记忆相关蛋白调节理论奠定了基础。
Objective To investigate the effects of simulated microgravity on the expression of memory function and learning-related proteins. Methods The 7 d tail suspension was used to simulate the micro-gravity environment. Memory mappings were detected by water maze and jumping platform. The expression of memory-related proteins, neuroprotective proteins and antioxidant enzymes were detected by Western blotting. The gene expression was detected by RT-qPCR. Results After 7 days’ tail suspension in SD rats, the spatial memory and location memory abilities decreased. Tail suspension decreased the activation of NR1 / 2B-CaMKII-CREB pathway in hippocampus of rats, and decreased the expression of Nrf2-mediated neuroprotective protein HO-1 by mechanical growth factor MGF. In addition, tails arrest the gene and protein expression of the antioxidant enzymes SOD2 and GPx. These results suggest that simulated microgravity significantly inhibits the NR1 / 2B and MGF signaling pathways in the hippocampus. Conclusions NR and MGF may serve as tail-suspended mediators of cognitive impairment and protective factors, respectively, which lays a foundation for the regulation of learning and memory-related proteins in cognitive impairment in microgravity.