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目的：探讨同型半胱氨酸（ＨＣＹ）对大鼠主动脉内皮细胞（ＥＣ）脂质过氧化的影响。方法：取体重１５０～２００ｇ健康雄性Ｗｉｓｔａｒ大鼠胸主动脉，以组织贴块法进行ＥＣ培养，第５代用于实验。各实验组及对照组均为 ６孔细胞，实验组加入 ５ｍｍｏｌ／Ｌ ＨＣＹ或 ５ ｍｍｏｌ／Ｌ ＨＣＹ＋ ２５μｍｏｌ／Ｌ Ｃｕ~２，对照组不加 ＨＣＹ。分别在不同时限观察细胞形态并测定丙二醛（ＭＤＡ）浓度，超氧化物歧化酶（ＳＯＤ）活性和乳酸脱氢酶（ＬＤＨ）浓度。结果：ＨＣＹ可引起ＥＣ形态变化，使培养液中ＬＤＨ及ＭＤＡ浓度增加，而ＳＯＤ活性下降，ＭＤＡ浓度与ＳＯＤ活性呈负相关（ｒ＝－０．８３８，Ｐ＜ ０． ０５），在有 Ｃｕ~２＋存在时，上述改变更为明显。结论： ＨＣＹ引起ＥＣ脂质过氧化可能是导致动脉粥样硬化的原因之一。
Objective: To investigate the effect of homocysteine (HCY) on lipid peroxidation in rat aortic endothelial cells (ECs). Methods: The thoracic aorta of healthy male Wistar rats weighing 150 ~ 200g were taken and cultured by tissue patch method. The fifth passage was used for the experiment. The experimental group and the control group were 6-hole cells, the experimental group by adding 5mmol / L HCY or 5 mmol / L HCY + 25μmol / L Cu ~ 2, the control group without HCY. The morphological changes of cells were observed at different time points and the concentrations of malondialdehyde (MDA), superoxide dismutase (SOD) and lactate dehydrogenase (LDH) were measured. Results: HCY could induce morphological changes of EC, increase the concentration of LDH and MDA, and decrease the activity of SOD in culture medium. The concentration of MDA was negatively correlated with the activity of SOD (r = -0.838, P <0.05) ~ 2 + exists, the above change is more obvious. Conclusions: HCY induced lipid peroxidation of EC may be one of the causes of atherosclerosis.