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目的研究RANTES基因启动子区-28C/G的多态性与子宫内膜异位症的关系。方法应用聚合酶链反应-限制性酶切片段长度多态性技术(PCR-RFLP)并进行测序的方法检测子宫内膜异位症患者46例(内异症组),非子宫内膜异位症患者35例(对照组)。结果子宫内膜异症组RANTES-28C/C、C/G两种基因型分布频率分别为84.78%、15.22%,对照组RANTES-28C/C、C/G两种基因型分布频率分别为85.71%、14.29%;两组间的组之间的基因型分布频率比较差异无显著性(P>0.05)。RANTES基因-28位点C、G等位基因型在内异症组中的分布频率分别为92.39%、7.61%,在对照组中分别为92.86%、7.14%,两组间等位基因型频率比较差异无统计学意义(P>0.05)。结论 RAN-TES启动子区-28C/G基因多态性与子宫内膜异位症遗传易感性无关联。
Objective To investigate the relationship between -28C / G polymorphism of RANTES gene promoter region and endometriosis. Methods 46 cases of endometriosis (endometriosis group), non-endometriosis (control group) and non-endometriosis group were detected by polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) 35 patients with disease (control group). Results The frequencies of RANTES-28C / C and C / G genotypes in endometriosis group were 84.78% and 15.22% respectively. The frequencies of RANTES-28C / C and C / G genotypes in control group were 85.71 %, 14.29% respectively. There was no significant difference in genotype frequencies between the two groups (P> 0.05). The distribution frequencies of RANTES gene -28 locus C and G allele in endometriosis group were 92.39% and 7.61%, respectively, which were 92.86% and 7.14% respectively in the control group. The allele frequencies The difference was not statistically significant (P> 0.05). Conclusion There is no correlation between -28C / G polymorphism of RAN-TES promoter region and genetic predisposition of endometriosis.