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目的 研究新决明内脂与细胞内脂质代谢有关的生物学效应及分子机理。方法 首先用动物实验检查该化合物的生物学效应。再用细胞学方法研究该化合物对 3T3 L1前脂肪细胞分化的作用。最后用含有 10 0 0 0个人类基因和ESTs的高密度cDNA微阵列去研究新决明内脂如何改变HepG2细胞的基因表达图谱。结果 新决明内脂可减少大鼠的体重增加率 ,但对 3T3 L1前脂肪细胞分化的作用有限。HepG2肝细胞基因表达图谱结果显示新决明内脂调节了 4 6个与脂质代谢、蛋白代谢、细胞增生与凋亡等功能有关的基因。结论 新决明内脂的生理效果可能与一系列与脂质代谢有关的重要基因有关。
Objective To study the biological effects and molecular mechanisms of Neocastrella lipids on intracellular lipid metabolism. Methods Animal experiments were used to examine the biological effects of this compound. The cytotoxicity of this compound on the differentiation of 3T3 L1 preadipocytes was also studied. Finally, a high-density cDNA microarray containing 10 000 human genes and ESTs was used to study how Neocatrel could alter the gene expression profile of HepG2 cells. Results New Cassia lipotica can reduce weight gain in rats, but limited the differentiation of 3T3 L1 preadipocytes. The gene expression profile of HepG2 hepatocytes showed that the new Cassia lipids regulated 46 genes involved in lipid metabolism, protein metabolism, cell proliferation and apoptosis. Conclusion The physiological effects of Neocarolipid may be related to a series of important genes related to lipid metabolism.