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目的:探讨锌-α2-糖蛋白(ZAG)在肥胖与正常SD大鼠肝脏中的表达情况。方法:采用高脂饮食方法建立雄性肥胖大鼠的动物模型,抽提肥胖和正常大鼠肝脏中的蛋白质,运用免疫印迹技术,检测ZAG的表达情况。结果:成功建立肥胖大鼠动物模型,ZAG在肥胖和正常大鼠肝脏中均有表达,其中肥胖大鼠肝脏表达量增加。结论:ZAG在肝脏中均有表达,尤其是在肥胖大鼠肝脏中表达较多,为进一步研究ZAG的功能提供必要的基础。
Objective: To investigate the expression of zinc-α2-glycoprotein (ZAG) in the liver of obese and normal SD rats. Methods: The animal model of male obese rats was established by high-fat diet. The protein in obese and normal rat liver was extracted and the expression of ZAG was detected by Western blotting. Results: The animal model of obese rats was successfully established. ZAG was expressed in the liver of obese and normal rats, and the expression of liver was increased in obese rats. CONCLUSION: ZAG is expressed in the liver, especially in the liver of obese rats, which provides the necessary foundation for further study on the function of ZAG.