选用继代培养７０ｄ的苹果离体茎尖，在５℃条件下低温驯化３周，经不同浓度蔗糖预培养及藻酸钠包埋后，在无菌空气中干燥脱水４ｈ直接投入液氮，化冻后茎尖存活率达７０％以上。通过选择材料的生理状态可缩短和简化保存过程。 The explants were subcultured for 70 days in vitro and acclimatized for 3 weeks at 5 ℃. After being pre-incubated with different concentrations of sucrose and embedded in sodium alginate, they were dehydrated in sterile air for 4 hours and then directly put into liquid nitrogen and thawed After the tip survival rate of 70%. By choosing the physical state of the material, the preservation process can be shortened and simplified.