论文部分内容阅读
在遗传毒性的检测中,细菌测试系统已为世界许多国家采用,因为它具有快速、简便等应用上的优点,并可提供遗传毒性的基本机制及其后果。细菌是含DNA的最简单的细胞,它拥有对DNA损伤剂作出反应的机制。在E.Coli中,已知有一套SOS反应功能(17个基因构成的SOS反应网络)在DNA损伤剂处理后作出表达。因此,可通过监测细菌细胞中SOS反应的方法以检测受试物的遗传毒性。近年来,已为多数学者应用于检测食品、有害气体、金属及其无机化合物、烷化剂和抗肿瘤药物的遗传毒性。本文即应用此法检测了七类14种消毒剂的遗传毒性。
In testing for genotoxicity, bacterial testing systems have been adopted in many countries around the world because of their rapid and simple application advantages and provide the basic mechanism of genotoxicity and its consequences. Bacteria are the simplest cells that contain DNA and have a mechanism that responds to DNA damaging agents. In E. coli, a set of SOS response functions (a 17-gene SOS reaction network) is known to be expressed after DNA damaging agent treatment. Thus, the genotoxicity of a test substance can be tested by monitoring the SOS response in bacterial cells. In recent years, most scholars have been applied to detect genotoxicity of foodstuffs, noxious gases, metals and their inorganic compounds, alkylating agents and anti-tumor drugs. This article is to apply this method to test the seven kinds of 14 kinds of disinfectants genotoxicity.