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目的:探讨宫血宁活性成分——重楼总皂苷(TSSP)诱导大鼠离体子宫肌条收缩的机制,考察PLA2/AA信号途径与TSSP引起的大鼠离体子宫收缩的关系。方法:应用离体子宫张力测定法,观察不同PLA2/AA信号途径抑制剂对TSSP引起的大鼠离体子宫收缩作用的影响。结果:非特异性前列腺素合成酶抑制剂可明显抑制TSSP和米索前列醇引起的子宫收缩,提示二者可能存在相似机制。环氧酶1(COX-1)抑制剂SC560,环氧酶2(COX-2)抑制剂NS-398,EP受体抑制剂AH6809和脂氧酶(5-LO)抑制剂REV5901均可对TSSP缩宫作用产生明显抑制;磷脂酶A2(PLA2)抑制剂AACOCF3也可明显抑制TSSP的缩宫作用,提示PLA2/AA信号途径与TSSP的缩宫作用密切相关。结论:TSSP对大鼠子宫平滑肌收缩活动的调节与PLA2/AA信号途径的激活有关。
Objective: To investigate the mechanism of the compound of Gongxuening, which is the active ingredient of schnexon, inducing the contraction of isolated uterine muscle strips in rats, and to investigate the relationship between PLA2/AA signal pathway and TSSP-induced isolated uterine contractions in rats. METHODS: The effects of different PLA2/AA signaling pathway inhibitors on isolated rat uterine contractions induced by TSSP were observed by ex vivo uterine tonometry. Results: Non-specific prostaglandin synthetase inhibitors significantly inhibited uterine contractions caused by TSSP and misoprostol, suggesting that there may be similar mechanisms. The cyclooxygenase 1 (COX-1) inhibitor SC560, the cyclooxygenase 2 (COX-2) inhibitor NS-398, the EP receptor inhibitor AH6809 and the lipoxygenase (5-LO) inhibitor REV5901 can both be used for TSSP The systolic effect was significantly inhibited; the phospholipase A2 (PLA2) inhibitor AACOCF3 also significantly inhibited the systolic effect of TSSP, suggesting that the PLA2/AA signal pathway is closely related to the systolic effect of TSSP. CONCLUSION: The regulation of contractile activity of rat uterine smooth muscle by TSSP is related to the activation of PLA2/AA signaling pathway.