目的　研究碱性成纤维细胞生长因子（ｂＦＧＦ） 在体外对牛晶状体上皮细胞（ＢＬＥＣ） 增殖的影响及确定ＢＬＥＣ 是否表达ｂＦＧＦ 蛋白。 方法　ＢＬＥＣ 原代培养。用不同浓度的ｂＦＧＦ 及３Ｈ胸腺嘧啶（３ＨＴＤＲ） 处理ＢＬＥＣ３６ ｈ 后，液闪测定ｂＦＧＦ 对ＢＬＥＣ３ＨＴＤＲ 掺入率的影响。Ｗｅｓｔｅｒｎ 印迹（ Ｗｅｓｔｅｒｎ ｂｌｏｔ） 法确定ＢＬＥＣ 是否表达ｂＦＧＦ 蛋白。 结果　ｂＦＧＦ 在体外有促进ＢＬＥＣ 增殖作用，Ｗｅｓｔｅｒｎ ｂｌｏｔ 法表明ＢＬＥＣ 可表达低分子量（１８ ０００）ｂＦＧＦ 蛋白。 结论　ｂＦＧＦ 可能通过晶状体上皮细胞的自分泌，促进白内障术后残留晶状体上皮细胞的增殖，导致后发性白内障 Objective To investigate the effect of basic fibroblast growth factor (bFGF) on the proliferation of bovine lens epithelial cells (BLECs) in vitro and to determine if BLECs express bFGF protein. Methods Primary culture of BLECs. After BLECs were treated with different concentrations of bFGF and 3H-thymidine (3HTDR) for 36 h, the influence of bFGF on the incorporation rate of BLEC3HTDR was determined by liquid scintillation. Western Blotting determines whether BLEC expresses bFGF protein. Results bFGF could promote the proliferation of BLEC in vitro. Western blot showed that BLEC could express low molecular weight (18 000) bFGF protein. Conclusion bFGF may promote the autocrine of lens epithelial cells and promote the proliferation of residual lens epithelial cells after cataract surgery,