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目的 研究类风湿关节炎 (RA)患者血清、滑液中白细胞介素 18(IL 18)蛋白及滑膜组织IL 18mRNA表达水平 ,探讨其在RA致病中的作用。方法 应用双抗夹心酶联免疫吸附(ELISA)法和细胞生物法分别测定RA患者血清、滑液中IL 18蛋白水平和生物活性 ,同时还检测NO、前列腺素E2 的含量 ;采用半定量RT PCR法检测滑膜组织IL 18mRNA表达水平。以骨关节炎(OA)病人及因外伤截肢的正常人作对照。结果 RA患者血清、滑液中IL 18蛋白水平和生物活性均显著高于对照组 ,滑液中量及活性比血清高 ;RA滑膜组织IL 18mRNA表达水平也明显高于对照组。结论 过度表达的IL 18参与了RA的致病过程 ;选择性地抑制IL 18生物活性 ,将是RA治疗的新途径。
Objective To investigate the expression of interleukin 18 (IL 18) and synovial IL 18mRNA in serum and synovial fluid of patients with rheumatoid arthritis (RA), and to explore its role in the pathogenesis of RA. Methods Serum and synovial fluid of patients with rheumatoid arthritis (RA) were collected. The levels of IL-18 protein and biological activity were measured by enzyme-linked immunosorbent assay (ELISA) and cell biology method. The contents of NO and prostaglandin E2 were also detected. Method to detect IL 18mRNA expression in synovial tissue. To osteoarthritis (OA) patients and traumatic amputation of normal controls. Results The levels of IL 18 protein and bioactivity in serum and synovial fluid of RA patients were significantly higher than those in control group, and the amount and activity of synovial fluid in serum were higher than those in serum. The expression level of IL 18 mRNA in RA synovium was also significantly higher than that in control group. Conclusion Overexpression of IL-18 is involved in the pathogenesis of RA. Selective inhibition of IL-18 bioactivity will be a new approach for the treatment of RA.