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目的 探讨质膜Ca2 + - ATP酶异构体 2 (PMCA2 )基因多态性与噪声性听力损失的关系。方法 采用横断面流行病学研究方法 ,对 194名噪声暴露作业工人进行调查和听力测试 ,按听力学评价的结果将其分为听力损失组和听力正常组 ;用多聚酶链反应 限制性片断长度多态性 (PCR -RFLP)方法和等位基因特异扩增法 (ASA)检测其PMCA2基因上rs2 2 892 74和rs6790 64 0两个单核苷酸位点的多态性。结果 在 93名噪声性听力损失的工人中 ,rs2 2 892 74位点AA、AG和GG基因型的频率分别为16. 1%、40. 9%和 43. 0 % ,等位基因A和G的频率为 3 6 6%和 63 . 4% ;在 10 1名听力正常的工人中 ,其基因型频率分别为 15 8%(AA)、3 2 7% (AG)和 5 .1 5 % (GG) ,等位基因频率为 3 2. 2 % (A)和 67. 8% (G)。rs6790 64 0位点在噪声性听力损失组CC、CT和TT基因型的频率分别为 0、 82 8%和 17. 2 % ,等位基因C和T的频率为 41. 4%和 5 8 .6% ;在听力正常组的基因型频率分别为 1 0 %(CC)、 76. 2 % (CT)和 2 2 . 8% (TT) ;等位基因频率为 3 9. 1% (C)和 60. 9% (T)。两位点的基因型分布及其等位基因频率在噪声性听力损失组与听力正常组之间差异均无显著性 (P >0 . 0 5 )。采用多元Logistic回归对两组间年龄、性别、吸烟状况、爆震史
Objective To investigate the relationship between plasma membrane Ca2 + - ATPase isoform 2 (PMCA2) gene polymorphism and noise-induced hearing loss. Methods A total of 194 noise exposure workers were surveyed and audiologically tested by the method of cross-sectional epidemiology. According to the results of audiological evaluation, they were divided into hearing loss group and normal hearing group. Polymerase chain reaction restriction fragment length PCR-RFLP and allele-specific amplification (ASA) were used to detect the SNPs of rs2 2 892 74 and rs6790 64 0 in PMCA2 gene. Results Among the 93 workers with noise-induced hearing loss, the frequencies of AA, AG and GG genotypes in rs2 2 892 74 loci were 16.1%, 40.9% and 43.0% respectively. The alleles A and G The frequencies of genotypes were 36.6% and 63.4%, respectively. Among 101 workers with normal hearing, the genotype frequencies were 15.8% (AA), 37.2% (AG) and 5.15% GG) with allele frequencies of 3.2% (A) and 67.8% (G). 4% 和 58. The frequency of CC, CT and TT genotypes of rs6790 64 0 locus in noise-induced hearing loss group were 0, 82 8% and 17 2%, respectively. The frequencies of alleles C and T were 41.4% and 58%. 6%; in the normal hearing group genotype frequency was 10% (CC), 76.2% (CT) and 22.8% (TT); allele frequency was 39.1% (C) And 60.9% (T). There was no significant difference in genotype distribution and allele frequencies between the two groups (P> 0.05) and normal hearing group (P> 0.05). Multivariate Logistic regression was used to analyze the age, sex, smoking status and detonation history between the two groups