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目的采用高效液相色谱-二极管阵列检测法同时测定消栓通胶囊中羟基红花黄色素A、芍药苷、阿魏酸、丹酚酸B、丹参酮ⅡA共5种活性成分的含量。方法采用Kromasil C18色谱柱(150 mm×4.6 mm,3.5μm);以甲醇-乙腈(体积比2∶1)(A)和体积分数0.1%磷酸水溶液(B)作流动相,流速1.0 mL.min-1,梯度洗脱;柱温30℃;检测波长羟基红花黄色素A为400 nm,芍药苷、阿魏酸为230 nm,丹酚酸B、丹参酮ⅡA为270 nm。结果羟基红花黄色素A、芍药苷、阿魏酸、丹酚酸B、丹参酮ⅡA的线性范围分别为5.16～51.6 mg·L-1(r=0.999 1)、26.52～265.2 mg·L-1(r=0.999 5)、2.04～20.4 mg·L-1(r=0.999 6)、28.20～282.0 mg·L-1(r=0.999 1)、2.40～24.0 mg·L-1(r=0.999 2),5种成分的平均加样回收率为98.2%～101.7%,RSD为0.9%～2.1%(n=6)。结论建立的方法专属、准确、重现,可用于消栓通胶囊的质量控制。
OBJECTIVE To determine the contents of five active ingredients of xanthochrysum A, paeoniflorin, ferulic acid, salvianolic acid B and tanshinone Ⅱ A in Xiaohuatong Capsules simultaneously by HPLC-diode array. Methods Kromasil C18 column (150 mm × 4.6 mm, 3.5 μm) was used as the mobile phase at a flow rate of 1.0 mL · min with methanol-acetonitrile (volume ratio 2: 1) (A) and 0.1% -1 with gradient elution. The column temperature was 30 ℃. The detection wavelength was 400 nm for hydroxysafflor yellow A, 230 nm for paeoniflorin and ferulic acid, and 270 nm for salvianolic acid B and tanshinone ⅡA. Results The linear ranges of safflower yellow pigment A, paeoniflorin, ferulic acid, salvianolic acid B and tanshinone ⅡA were 5.16-51.6 mg · L -1 (r = 0.999 1), 26.52-265.2 mg · L -1 (r = 0.999 5), 2.04-20.4 mg · L -1 (r = 0.999 6), 28.20-2282.0 mg · L -1 (r = 0.999 1), 2.40-24.0 mg · L -1 (r = 0.999 2) ). The average recoveries of five components were 98.2% ~ 101.7% with RSD of 0.9% ~ 2.1% (n = 6). Conclusion The established method is specific, accurate and reproducible and can be used for the quality control of Xiaobu Tong capsule.