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本文观察了肺癌和良性肺疾病胸水的涎酸期特异性鼠胚抗原(涎酸 SSEA-1)水平,并探讨其诊断意义。肺癌患者38(男26、女12)例,年龄27~87岁,其中腺癌25例、鳞癌3例、小细胞癌5例、大细胞癌3例、转移癌2例。良性肺病变13(男8、女5)例,年龄17~82岁,包括结核8例,脓胸2例、气胸3例。采集的胸水标本离心后冷藏于-20℃。采用 FH6单克隆抗体固相免疫夹层试验,测定涎酸 SSEA-1;用酶免疫法测定癌胚抗原(CEA)。
In this paper, the citrate-phase specific murine embryonic antigen (citric acid SSEA-1) levels in pleural effusions of lung cancer and benign lung diseases were observed and their diagnostic significance was explored. There were 38 patients (26 males and 12 females) with lung cancer, aged 27-87 years, including 25 adenocarcinomas, 3 squamous cell carcinomas, 5 small cell carcinomas, 3 large cell carcinomas, and 2 metastatic carcinomas. Benign lung lesions 13 (male 8, female 5) cases, aged 17 to 82 years old, including 8 cases of tuberculosis, 2 cases of empyema, 3 cases of pneumothorax. The collected pleural fluid specimens were refrigerated at -20°C after centrifugation. The solid phase immuno sandwich assay with FH6 monoclonal antibody was used to determine the citric acid SSEA-1. Carcinoembryonic antigen (CEA) was measured by enzyme immunoassay.