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[目的 ]观察无损伤的中等强度的习服声刺激后耳蜗中热应激蛋白 (heatshockprotein ,HSP)的表达特点。 [方法 ]习服组先接受 10d每天 6h习服声暴露 ;对照组静置饲养 10d ;习服 +高噪声暴露组先接受 10d每天 6h习服声暴露 ,休息 2d后 ,再暴露于高强度噪声 2h ;高噪声暴露组直接暴露于高强度噪声 2h。然后以免疫组织化学方法结合图像分析技术检测各组动物耳蜗HSP70表达情况。 [结果 ]习服组耳蜗中HSP70 (积分光密度为 2 2 .99,平均光密度为 0 .2 8)明显高于对照组 (积分光密度为 6.77,平均光密度为 0 .11) (P <0 .0 5 ) ;习服 +高噪声组明显低于高噪声暴露组 (P <0 .0 5 ) ,两组积分光密度分别为 2 0 .0 6和 43 .14 ,平均光度分别为 0 .3 0和 0 .40。 [结论 ]用无损伤的中等强度习服声诱导耳蜗中HSP70的表达高于正常状态时的表达而低于高强度噪声刺激时的表达 ,但习服过程诱导产生的HSP70却对听觉系统提供了一定的保护作用
[Objective] To observe the expression characteristics of heat shock protein (HSP) in the cochlea of non-injured moderate-intensity acoustical stimuli. [Methods] The accustom group received daily 6h acclimatization sound exposure for 10 days, while the control group was kept for 10 days. The acclimation + high noise exposure group was exposed for 6h with daily accupuncture exposure for 10 days and then exposed to high intensity noise for 2 hours after resting for 2 days. The exposed group was directly exposed to high intensity noise for 2h. Then immunohistochemistry and image analysis techniques were used to detect the expression of HSP70 in each group of animals. [Results] HSP70 (integrated optical density was 22.999, average optical density was 0.828) in the cochlear of the acclimation group was significantly higher than that of the control group (integral optical density was 6.77, average optical density was 0.11) (P <0. 05). The acclimation + high noise group was significantly lower than that of the high noise exposure group (P <0.05). The integral optical densities of the two groups were 20.06 and 43.14 respectively, and the average luminosities were 0 .3 0 and 0 .40. [Conclusion] The expression of HSP70 in cochlea induced by moderate-intensity acupuncture with no injury is higher than that in normal condition and lower than that in high-intensity noise stimulation. However, HSP70 induced by acclimation process may provide certain Protective effects