目的:研究不同采摘时期连翘叶的总黄酮、总酚酸含量的变化及其与清除2,2-二苯基-1-苦肼基自由基(2,2-diphenyl-1-picrylhydrazyl,DPPH)能力的关系。方法:连翘叶样品粉末脱脂后用60%甲醇超声波提取。采用DPPH自由基清除法评价不同采摘时期连翘叶的抗氧化能力。样品粗提取物总黄酮含量的测定采用亚硝酸钠法,总酚酸含量的测定采用Folin试剂法。采用SPSS 13.0分析软件对数据进行统计学处理。结果:不同采摘时期连翘叶的总黄酮、总酚酸含量以及清除DPPH自由基的能力存在着显著的差异;3月采摘的刚萌发幼叶表现出最高的总黄酮、总酚酸含量以及清除DPPH自由基的能力。相关性研究表明,连翘叶的总黄酮、总酚酸含量与清除DPPH自由基能力均存在显著正相关关系,其相关系数分别为0.886,0.841。结论:3月连翘叶的抗氧化能力和抗氧化物质含量都显著高于其他生长时期连翘叶,这显示从抗氧化方面而言连翘嫩叶刚萌发的时期是最佳的采摘时期,为较好的开发和利用连翘叶提供了理论依据。 OBJECTIVE: To study the changes of total flavonoids and total phenolic acids in Forsythia suspense (Forsythia suspense) leaves and their correlations with 2,2-diphenyl-1-picrylhydrazyl (DPPH) ) The relationship between ability. Methods: Forsythia suspensa leaf samples were degreased with 60% methanol. DPPH radical scavenging assay was used to evaluate the antioxidative capacity of Forsythia suspensa leaves at different harvesting times. The crude extract of the sample was assayed for total flavonoids by the sodium nitrite method, and the total phenolic acid content was determined by Folin’s reagent method. The data were statistically processed using SPSS 13.0 analysis software. Results: There were significant differences in total flavonoids, total phenolic acids and DPPH scavenging ability of Forsythia suspensa leaves at different picking times. The highest content of total flavonoids, total phenolic acids and total phenolic acids in freshly picked leaves were observed in March DPPH free radical capacity. Correlation studies have shown that the total flavonoids, total phenolic acids content of Forsythia suspensa leaves and DPPH radical scavenging capacity have a significant positive correlation, the correlation coefficients were 0.886,0.841. CONCLUSION: The antioxidant capacity and anti-oxidant content of Forsythia suspensa leaves in March were significantly higher than that of Forsythia suspense leaves in other growth stages, which indicated that the young leaves of Forsythia suspensa had the best germination period from the aspects of antioxidation, For the better development and utilization of Forsythia provide the theoretical basis.